Western Blot:Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes.
Article Snippet: The integrated density of the blot bands was quantified and analyzed using ImageJ and Prism 6.0 software to assess the relative protein levels. .. The primary antibodies used in western blot assays are as follows: Munc13-4 Antibody (C-2) (Santa Cruz, sc-271300, 1:1000, for samples of human origin), UNC13D Monoclonal antibody (Proteintech, 67193-1-Ig, 1:5000, for samples ofmouse origin), PD-L1/CD274 RabbitmAb (Abclonal, A19135, 1:2000, for samples of human origin), Anti-PD-L1 antibody (abcam, ab213480, 1:2000, for samples of mouse origin), HGS Polyclonal antibody (Proteintech, 10390-1-AP, 1:10000, for samples of both human and mouse origin), β-Actin Rabbit mAb (High Dilution) (Abclonal, AC026, 1:100000, for samples of both human and mouse origin), Alix Monoclonal antibody (Proteintech, 67715-1-Ig, 1:5000, for samples of both human and mouse origin), CD63 Antibody (MX-49.129.5) (Santa Cruz, sc-5275, 1:1000, for samples of both human and mouse origin), CD81 Antibody (B-11) (Santa Cruz, sc-166029, 1:1000, for samples of both human and mouse origin), Mouse anti-GFP-Tag mAb (Abclonal, AE012, 1:10000, species independent), Rabbit anti-GFP-Tag pAb (Abclonal, AE011, 1:10000, species independent), DYKDDDDK tag Polyclonal antibody (Proteintech, 20543-1-AP, 1:10000, species independent), StrepII Tag Mouse Monoclonal Antibody (Beyotim, AF2924, 1:1000, species independent), Mouse anti HA-Tag mAb (Abclonal, AE008, 1:5000, species independent), Pan Acetylation Monoclonal antibody (Proteintech, 66289-1-Ig, 1:1000, species independent), Ubiquitin Antibody (P4D1) (Santa Cruz, sc-8017, 1:1000, for samples of both humanandmouseorigin), panPhospho-Serine/Threonine Rabbit Polyclonal Antibody (Beyotim, AF5725, 1:1000, species independent), Pan Phospho-Tyrosine Mouse mAb (Abclonal, AP0973, 1:1000, for samples of both human and mouse origin), CBP/KAT3A/CREBBP Antibody (C-1) (Santa Cruz, sc-7300, 1:1000, for samples of both human andmouse origin), P300 Antibody (F-4) (Santa Cruz, sc-48343, 1:1000, for samples of both human and mouse origin), Histone Deacetylase 3 (HDAC3) Antibody (A-3) (Santa Cruz, sc-376957, 1:1000, for samples of both human and mouse origin), Histone Deacetylase 4 (HDAC4)Antibody (B-5) (Santa Cruz, sc-365093, 1:1000, for samples of both human and mouse origin), NEDD4L Polyclonal antibody (Proteintech, 13690-1-AP, 1:3000, for samples of both human and mouse origin). .. The secondary antibodies used in western blot experiments are as follows: HRP-conjugated Goat anti-Rabbit IgG (H+L) (Abclonal, AS014, 1:10000), HRP-conjugated Goat anti-Mouse IgG (H+L) Nature Communications | (2025) 16:9080 17 (Abclonal, AS003, 1:10000).
Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes
Article Snippet: The integrated density of the blot bands was quantified and analyzed using ImageJ and Prism 6.0 software to assess the relative protein levels. .. The primary antibodies used in western blot assays are as follows: Munc13-4 Antibody (C-2) (Santa Cruz, sc-271300, 1:1000), PD-L1/CD274 Rabbit mAb (Abclonal, A19135, 1:2000), HGS Polyclonal antibody (Proteintech, 10390-1-AP, 1:10000), β-Actin Rabbit mAb (High Dilution) (Abclonal, AC026, 1:100000), Alix Monoclonal antibody (Proteintech, 67715-1-Ig, 1:5000), CD63 Antibody (MX-49.129.5) (Santa Cruz, sc-5275, 1:1000), CD81 Antibody (B-11) (Santa Cruz, sc-166029, 1:1000), Mouse anti GFP-Tag mAb (Abclonal, AE012, 1:10000), Rabbit anti GFP-Tag pAb (Abclonal, AE011, 1:10000), DYKDDDDK tag Polyclonal antibody (Proteintech, 20543-1-AP, 1:10000), StrepII Tag Mouse Monoclonal Antibody (Beyotim, AF2924, 1:1000), Mouse anti HA-Tag mAb (Abclonal, AE008, 1:5000), Pan Acetylation Monoclonal antibody (Proteintech, 66289-1-Ig, 1:1000), Ubiquitin Antibody (P4D1) (Santa Cruz, sc-8017, 1:1000), pan Phospho-Serine/Threonine Rabbit Polyclonal Antibody (Beyotim, AF5725, 1:1000), Pan Phospho-Tyrosine Mouse mAb (Abclonal, AP0973, 1:1000), CBP/KAT3A/CREBBP Antibody (C-1) (Santa Cruz, sc-7300, 1:1000), P300 Antibody (F-4) (Santa Cruz, sc-48343, 1:1000), Histone Deacetylase 3 (HDAC3) Antibody (A-3) (Santa Cruz, sc-376957, 1:1000), Histone Deacetylase 4 (HDAC4) Antibody (B-5) (Santa Cruz, sc-365093, 1:1000). .. The secondary antibodies used in western blot experiments are as follows: HRP-conjugated Goat anti- Rabbit IgG (H+L) (Abclonal, AS014, 1:10000), HRP-conjugated Goat anti-Mouse IgG (H+L) (Abclonal, AS003, 1:10000).
Ubiquitin Proteomics:Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes.
Article Snippet: The integrated density of the blot bands was quantified and analyzed using ImageJ and Prism 6.0 software to assess the relative protein levels. .. The primary antibodies used in western blot assays are as follows: Munc13-4 Antibody (C-2) (Santa Cruz, sc-271300, 1:1000, for samples of human origin), UNC13D Monoclonal antibody (Proteintech, 67193-1-Ig, 1:5000, for samples ofmouse origin), PD-L1/CD274 RabbitmAb (Abclonal, A19135, 1:2000, for samples of human origin), Anti-PD-L1 antibody (abcam, ab213480, 1:2000, for samples of mouse origin), HGS Polyclonal antibody (Proteintech, 10390-1-AP, 1:10000, for samples of both human and mouse origin), β-Actin Rabbit mAb (High Dilution) (Abclonal, AC026, 1:100000, for samples of both human and mouse origin), Alix Monoclonal antibody (Proteintech, 67715-1-Ig, 1:5000, for samples of both human and mouse origin), CD63 Antibody (MX-49.129.5) (Santa Cruz, sc-5275, 1:1000, for samples of both human and mouse origin), CD81 Antibody (B-11) (Santa Cruz, sc-166029, 1:1000, for samples of both human and mouse origin), Mouse anti-GFP-Tag mAb (Abclonal, AE012, 1:10000, species independent), Rabbit anti-GFP-Tag pAb (Abclonal, AE011, 1:10000, species independent), DYKDDDDK tag Polyclonal antibody (Proteintech, 20543-1-AP, 1:10000, species independent), StrepII Tag Mouse Monoclonal Antibody (Beyotim, AF2924, 1:1000, species independent), Mouse anti HA-Tag mAb (Abclonal, AE008, 1:5000, species independent), Pan Acetylation Monoclonal antibody (Proteintech, 66289-1-Ig, 1:1000, species independent), Ubiquitin Antibody (P4D1) (Santa Cruz, sc-8017, 1:1000, for samples of both humanandmouseorigin), panPhospho-Serine/Threonine Rabbit Polyclonal Antibody (Beyotim, AF5725, 1:1000, species independent), Pan Phospho-Tyrosine Mouse mAb (Abclonal, AP0973, 1:1000, for samples of both human and mouse origin), CBP/KAT3A/CREBBP Antibody (C-1) (Santa Cruz, sc-7300, 1:1000, for samples of both human andmouse origin), P300 Antibody (F-4) (Santa Cruz, sc-48343, 1:1000, for samples of both human and mouse origin), Histone Deacetylase 3 (HDAC3) Antibody (A-3) (Santa Cruz, sc-376957, 1:1000, for samples of both human and mouse origin), Histone Deacetylase 4 (HDAC4)Antibody (B-5) (Santa Cruz, sc-365093, 1:1000, for samples of both human and mouse origin), NEDD4L Polyclonal antibody (Proteintech, 13690-1-AP, 1:3000, for samples of both human and mouse origin). .. The secondary antibodies used in western blot experiments are as follows: HRP-conjugated Goat anti-Rabbit IgG (H+L) (Abclonal, AS014, 1:10000), HRP-conjugated Goat anti-Mouse IgG (H+L) Nature Communications | (2025) 16:9080 17 (Abclonal, AS003, 1:10000).
Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes
Article Snippet: The integrated density of the blot bands was quantified and analyzed using ImageJ and Prism 6.0 software to assess the relative protein levels. .. The primary antibodies used in western blot assays are as follows: Munc13-4 Antibody (C-2) (Santa Cruz, sc-271300, 1:1000), PD-L1/CD274 Rabbit mAb (Abclonal, A19135, 1:2000), HGS Polyclonal antibody (Proteintech, 10390-1-AP, 1:10000), β-Actin Rabbit mAb (High Dilution) (Abclonal, AC026, 1:100000), Alix Monoclonal antibody (Proteintech, 67715-1-Ig, 1:5000), CD63 Antibody (MX-49.129.5) (Santa Cruz, sc-5275, 1:1000), CD81 Antibody (B-11) (Santa Cruz, sc-166029, 1:1000), Mouse anti GFP-Tag mAb (Abclonal, AE012, 1:10000), Rabbit anti GFP-Tag pAb (Abclonal, AE011, 1:10000), DYKDDDDK tag Polyclonal antibody (Proteintech, 20543-1-AP, 1:10000), StrepII Tag Mouse Monoclonal Antibody (Beyotim, AF2924, 1:1000), Mouse anti HA-Tag mAb (Abclonal, AE008, 1:5000), Pan Acetylation Monoclonal antibody (Proteintech, 66289-1-Ig, 1:1000), Ubiquitin Antibody (P4D1) (Santa Cruz, sc-8017, 1:1000), pan Phospho-Serine/Threonine Rabbit Polyclonal Antibody (Beyotim, AF5725, 1:1000), Pan Phospho-Tyrosine Mouse mAb (Abclonal, AP0973, 1:1000), CBP/KAT3A/CREBBP Antibody (C-1) (Santa Cruz, sc-7300, 1:1000), P300 Antibody (F-4) (Santa Cruz, sc-48343, 1:1000), Histone Deacetylase 3 (HDAC3) Antibody (A-3) (Santa Cruz, sc-376957, 1:1000), Histone Deacetylase 4 (HDAC4) Antibody (B-5) (Santa Cruz, sc-365093, 1:1000). .. The secondary antibodies used in western blot experiments are as follows: HRP-conjugated Goat anti- Rabbit IgG (H+L) (Abclonal, AS014, 1:10000), HRP-conjugated Goat anti-Mouse IgG (H+L) (Abclonal, AS003, 1:10000).
Histone Deacetylase Assay:Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes.
Article Snippet: The integrated density of the blot bands was quantified and analyzed using ImageJ and Prism 6.0 software to assess the relative protein levels. .. The primary antibodies used in western blot assays are as follows: Munc13-4 Antibody (C-2) (Santa Cruz, sc-271300, 1:1000, for samples of human origin), UNC13D Monoclonal antibody (Proteintech, 67193-1-Ig, 1:5000, for samples ofmouse origin), PD-L1/CD274 RabbitmAb (Abclonal, A19135, 1:2000, for samples of human origin), Anti-PD-L1 antibody (abcam, ab213480, 1:2000, for samples of mouse origin), HGS Polyclonal antibody (Proteintech, 10390-1-AP, 1:10000, for samples of both human and mouse origin), β-Actin Rabbit mAb (High Dilution) (Abclonal, AC026, 1:100000, for samples of both human and mouse origin), Alix Monoclonal antibody (Proteintech, 67715-1-Ig, 1:5000, for samples of both human and mouse origin), CD63 Antibody (MX-49.129.5) (Santa Cruz, sc-5275, 1:1000, for samples of both human and mouse origin), CD81 Antibody (B-11) (Santa Cruz, sc-166029, 1:1000, for samples of both human and mouse origin), Mouse anti-GFP-Tag mAb (Abclonal, AE012, 1:10000, species independent), Rabbit anti-GFP-Tag pAb (Abclonal, AE011, 1:10000, species independent), DYKDDDDK tag Polyclonal antibody (Proteintech, 20543-1-AP, 1:10000, species independent), StrepII Tag Mouse Monoclonal Antibody (Beyotim, AF2924, 1:1000, species independent), Mouse anti HA-Tag mAb (Abclonal, AE008, 1:5000, species independent), Pan Acetylation Monoclonal antibody (Proteintech, 66289-1-Ig, 1:1000, species independent), Ubiquitin Antibody (P4D1) (Santa Cruz, sc-8017, 1:1000, for samples of both humanandmouseorigin), panPhospho-Serine/Threonine Rabbit Polyclonal Antibody (Beyotim, AF5725, 1:1000, species independent), Pan Phospho-Tyrosine Mouse mAb (Abclonal, AP0973, 1:1000, for samples of both human and mouse origin), CBP/KAT3A/CREBBP Antibody (C-1) (Santa Cruz, sc-7300, 1:1000, for samples of both human andmouse origin), P300 Antibody (F-4) (Santa Cruz, sc-48343, 1:1000, for samples of both human and mouse origin), Histone Deacetylase 3 (HDAC3) Antibody (A-3) (Santa Cruz, sc-376957, 1:1000, for samples of both human and mouse origin), Histone Deacetylase 4 (HDAC4)Antibody (B-5) (Santa Cruz, sc-365093, 1:1000, for samples of both human and mouse origin), NEDD4L Polyclonal antibody (Proteintech, 13690-1-AP, 1:3000, for samples of both human and mouse origin). .. The secondary antibodies used in western blot experiments are as follows: HRP-conjugated Goat anti-Rabbit IgG (H+L) (Abclonal, AS014, 1:10000), HRP-conjugated Goat anti-Mouse IgG (H+L) Nature Communications | (2025) 16:9080 17 (Abclonal, AS003, 1:10000).
Article Title: Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes
Article Snippet: The integrated density of the blot bands was quantified and analyzed using ImageJ and Prism 6.0 software to assess the relative protein levels. .. The primary antibodies used in western blot assays are as follows: Munc13-4 Antibody (C-2) (Santa Cruz, sc-271300, 1:1000), PD-L1/CD274 Rabbit mAb (Abclonal, A19135, 1:2000), HGS Polyclonal antibody (Proteintech, 10390-1-AP, 1:10000), β-Actin Rabbit mAb (High Dilution) (Abclonal, AC026, 1:100000), Alix Monoclonal antibody (Proteintech, 67715-1-Ig, 1:5000), CD63 Antibody (MX-49.129.5) (Santa Cruz, sc-5275, 1:1000), CD81 Antibody (B-11) (Santa Cruz, sc-166029, 1:1000), Mouse anti GFP-Tag mAb (Abclonal, AE012, 1:10000), Rabbit anti GFP-Tag pAb (Abclonal, AE011, 1:10000), DYKDDDDK tag Polyclonal antibody (Proteintech, 20543-1-AP, 1:10000), StrepII Tag Mouse Monoclonal Antibody (Beyotim, AF2924, 1:1000), Mouse anti HA-Tag mAb (Abclonal, AE008, 1:5000), Pan Acetylation Monoclonal antibody (Proteintech, 66289-1-Ig, 1:1000), Ubiquitin Antibody (P4D1) (Santa Cruz, sc-8017, 1:1000), pan Phospho-Serine/Threonine Rabbit Polyclonal Antibody (Beyotim, AF5725, 1:1000), Pan Phospho-Tyrosine Mouse mAb (Abclonal, AP0973, 1:1000), CBP/KAT3A/CREBBP Antibody (C-1) (Santa Cruz, sc-7300, 1:1000), P300 Antibody (F-4) (Santa Cruz, sc-48343, 1:1000), Histone Deacetylase 3 (HDAC3) Antibody (A-3) (Santa Cruz, sc-376957, 1:1000), Histone Deacetylase 4 (HDAC4) Antibody (B-5) (Santa Cruz, sc-365093, 1:1000). .. The secondary antibodies used in western blot experiments are as follows: HRP-conjugated Goat anti- Rabbit IgG (H+L) (Abclonal, AS014, 1:10000), HRP-conjugated Goat anti-Mouse IgG (H+L) (Abclonal, AS003, 1:10000).
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